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7076986 
Journal Article 
Cresyl violet: a red fluorescent Nissl stain 
Alvarez-Buylla, A; Ling, CY; Kirn, , JR; , 
1990 
Yes 
Journal of Neuroscience Methods
ISSN: 0165-0270 
English 
2232864 
Cresyl violet is widely used by neurobiologists to visualize Nissl substance in bright-field microscopy. Here we describe a method for using this dye as a red fluorescent Nissl stain. Unlike the bright-field staining technique, fluorescent cresyl is compatible with other fluorescent dyes and tracers, such as fluorescein, Fluoro-Gold and Fast Blue. The procedure requires only minor modifications of routine bright-field cresyl staining, the most significant being dilution of the stain. Thus, fluorescent red cresyl violet is simple to implement and may be of general use in fluorescence microscopy.