Health & Environmental Research Online (HERO)


Print Feedback Export to File
7077581 
Journal Article 
MULTIPLEX PCR OF BCR-ABL FUSION TRANSCRIPTS IN PHILADELPHIA-POSITIVE ACUTE LYMPHOBLASTIC-LEUKEMIA 
Lee, A; Kirk, J; Edmands, S; Radich, J; , 
1995 
COLD SPRING HARBOR LAB PRESS 
PLAINVIEW 
283-287 
7580915 
WOS:A1995RM62900006 
We describe a multiplex PCR assay for the detection of bcr-abl fusion mRNA in Philadelphia chromosome positive acute lymphoblastic leukemia (Ph+ ALL), The assay provides a quick method for screening p190 (e1:a2) and p210 (b2:a2 or b3:a2) bcr-abl mRNAs simultaneously. The assay proves to be highly sensitive with detection of as little as one positive bcr-abl-expressing cell in a background of 10(5) negative bcr-abl cells. Bone marrow and peripheral blood specimens from six patients were in total accordance when run by multiplex PCR and by the single primer PCR approach. The multiplex bcr-abl assay may grove to be highly useful for screening newly diagnosed patients with ALL for the bcr-abl fusion transcript and in following the course of disease during therapy.