Health & Environmental Research Online (HERO)


Print Feedback Export to File
7095951 
Journal Article 
Experimental comparison of sonoporation and electroporation in cell transfection applications 
Pepe, J; Rincon, M; Wu, J; , 
2004 
ACOUSTICAL SOC AMER AMER INST PHYSICS 
MELVILLE 
62-67 
WOS:000220095600010 
An experimental study has been performed for cell suspensions to compare efficiency of cell transfection. It was demonstrated that electroporation was superior to sonoporation in terms of viability (65.8+/-2.3% vs 50.8+/-4.15%) and transfection efficiency (15.83+/-3.5% vs 7.53+/-0.4%) for Jurkat lymphocytes (nonprimary cells), and sonoporation was better in terms of viability (64.8+/-1.51% vs 53.7+/-1.53%) and transfection efficiency (2.73+/-0.21% vs 0.43+/-0.06%) for human peripheral blood mononuclear cells ( primary cells). The electroporation was performed using a Gene Pulser(R) II Apparatus with voltage of 250 V, and the sonoporation was achieved using 2-MHz pulsed ultrasound exposure (I-SPPA=80 W/cm(2)) assisted with encapsulated bubbles (Optison(R)). (C) 2004 Acoustical Society of America. 
• PFAS Universe
     Data Source
          Web of Science
     Perflutren