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HERO ID
7099516
Reference Type
Journal Article
Title
Identification of LL and Meso-diaminopimelic acid (DAP) of Actinomycete strains and Its Secondary Metabolite Production
Author(s)
Andayani, DGSri; Agustine, S; Mulyati, Y; Dewi, PL; Hakiki, M; ,
Year
2015
Publisher
ELSEVIER SCIENCE BV
Location
AMSTERDAM
Page Numbers
451-464
DOI
10.1016/j.proche.2015.12.078
Web of Science Id
WOS:000380833300066
Abstract
Streptomyces and Nocardia is a genus of actinomycetes group which has some similar characteristic. Although all of them have the ability to produce antibiotics but almost of Nocardia is pathogenic. The objective of this study is analyzed the two strains of actinomycetes and determine the optimum conditions of production of secondary metabolites using Streptomyces sp. Identification to distinguish the two strains (TP2 and TP5) by analyzing LL and meso-DAP on their cell walls. Optimization of the fermentation process using one factor at one time (OFAT) method that conducted at temperature of 26-40 degrees C, pH 5-8 and 120-180 rpm for 48 hours in a fermentor working volume 1 L. Antibacterial activity test performed by the agar diffusion method against resistant pathogenic bacteria. The results showed Streptomyces sp. TP2, contain any form of LL-DAP and Nocardia sp. TP5, and contain any form of meso-DAP as its main component. Optimization of antibiotic production is reached after fermentation be held for 42 hours at a temperature of 30 degrees C, pH 7 and 180 rpm with antibacterial activity against methicillin-sensitive Staphylococcus aureus, methicillin resistant Staphylococcus aureus, methicillin resistant coagulase negative Staphylococcus, vancomycin resistant Enterococcus, and Escherichia colt. (C) 2015 Published by Elsevier B.V.
Editor(s)
Tursiloadi, S; Hanafi, M; Sudiyani, Y;
Conference Name
International Symposium on Applied Chemistry
Conference Location
Bandung, INDONESIA
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