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7099907 
Journal Article 
Isolation and Characterization of Biotechnologically Potent Micrococcus luteus Strain From Environment 
Akbar, Ali; Sitara, U; Ali, I; Muhammad, N; Khan, SA; , 
2014 
1 
Pakistan Journal of Zoology
ISSN: 0030-9923 
ZOOLOGICAL SOC PAKISTAN 
LAHORE 
967-973 
WOS:000341675800011 
This study was conducted to isolate antagonistic and biotechnologically potent bacteria from environment. Micrococcus luteus was the only bacterium found producing antimicrobial metabolites and exhibited good probiotic characteristics. Micrococcus luteus showed antibacterial activity against foodborne pathogens, Salmonella typhimurium (16-20 mm), Listeria monocytogenes (11-15 mm) and Escherichia colt (5-10 mm). The bacterium was also found active against Klebsiella pneumoniae and Staphylococcus aureus. It was found producing industrially important enzymes including protease, lipase and phytase, confirmed by screening with specific substrate supplementations. The bacterium was able to survive in 2% bile salt and 4% NaCl with good probiotic potential. The DPPH radical scavenging activity of the Micrococcus luteus was found 79.61 +/- 0.02%, while the total phenolic content was 200 mu g/mL equivalent to gallic acid.