Health & Environmental Research Online (HERO)


Print Feedback Export to File
7101583 
Journal Article 
Effects of Escherichia coli infection and Eimeria tenella invasion on blood concentrations of some positive acute phase proteins (haptoglobin (PIT 54), fibrinogen and ceruloplasmin) in chickens 
Georgieva, TM; Georgiev, IP; Koinarski, ZV; Koinarski, VN; Urumova, VS; Marutsov, PD; Christov, TT; Nikolov, J; Chaprazov, T; Walshe, K; Karov, RS; , 
2010 
1 
Revue de Medecine Veterinaire
ISSN: 0035-1555 
ECOLE NATIONALE VETERINAIRE TOULOUSE 
TOULOUSE CEDEX 3 
84-89 
WOS:000276433300007 
Infections with enteropathogen E. coli and E. tenella commonly cause gastrointestinal inflammatory disorders in chickens. The present study was conducted to evaluate the changes in the plasma concentrations of haptoglobin-like protein (PIT54), ceruloplasmin and fibrinogen during separate or combined infections with E. coli and E. tenella. For that, 40 bred Cobb 500 chickens were randomly allotted into 4 equal groups according to the infection modalities: a first group was orally infected with E. tenella oocysts (8.10(4)) when birds were 12 days old, a second group was intraperitoneally inoculated with serotype 078 E. colt (10(7) cfu) when birds were 16 days old, a third group received the 2 combined infections whereas the last group serves as negative controls. Blood plasma samples were collected when chickens were 20 days old. The plasma concentrations of the 3 biochemical markers were dramatically increased in birds infected with E. coli alone or in association with E. tenella (compared to controls, P < 0.001). The chickens treated with Eimeria alone also exhibited significant but moderate increases of plasma ceruloplasmin and fibrinogen concentrations (P < 0.001) whereas the PIT 54 concentrations did not significantly vary in this group although they tended to be elevated compared to the not infected controls. Moreover, a positive correlation was evidenced between haptoglobin and fibrinogen concentrations (r = 0.56, P < 0.05) in all birds receiving Eimeria alone or combined with the bacteria. These results demonstrate that ceruloplasmin, fibrinogen and PIT 54 at a lesser extend are major positive acute phase proteins in chickens suffering from inflammation from an infectious origin and they also suggest that the intensity of the acute phase response greatly depends from the nature of the inflammatory stimulus.