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7103238 
Journal Article 
Analysis of Protein Phosphorylation Using Phos-Tag Gels 
Nagy, Z; Comer, S; Smolenski, A; , 
2018 
0 
Current Protocols in Protein Science
ISSN: 1934-3655 
English 
30044546 
Phos-tag gels are recent tools to dissect protein phosphorylation that operate by inducing a shift in the electrophoretic mobility of phosphorylated proteins compared to their nonphosphorylated counterparts. This article describes the preparation and electrophoresis of Zn2+ -Phos-tag gels along with electrotransfer of the separated phospho- and nonphosphoproteins onto a PVDF membrane using either wet-tank or semidry transfer. We also discuss the theory behind the technology with critical parameters to keep in mind for its successful application. © 2018 by John Wiley & Sons, Inc.