US EPA

7125717 

Journal Article 

Convenient preparation of 2?-O-rhamnosyl icariside II, a rare secondary flavonol glycoside, by recyclable and integrated biphase enzymatic hydrolysis 

Feng, C; Lu, Yi; Zhou, Y; Pang, Hui; Shen, Y; Yang, H; , 

2019 

1 

Pharmacognosy Magazine
ISSN: 0973-1296 

WOLTERS KLUWER MEDKNOW PUBLICATIONS 

MUMBAI 

147-155 

10.4103/pm.pm_398_18 

WOS:000456610600021 

Background: 2?-O-rhamnosyl icariside II, a rare secondary flavonol glycoside in Epimedii Folium (EF), has much better in vivo bioactivities than its original glycoside epimedin C. Its preparation methods, such as acidic hydrolysis, are of low efficiency, with by-products generated. Objective: The objective of this study was to establish a novel catalysis system for convenient preparation of this compound based on the recyclable and integrated biphase enzymatic hydrolysis. Materials and Methods: -dextranase was selected from five commercial enzymes due to the best catalysis performance. After optimization of conditions, the biphase system was constructed with propyl acetate and HAc-NaAc buffer (pH 4.5) (3:2, v/v) containing -dextranase/epimedin C (1:2, w/w), and the hydrolysis was performed at 60 degrees C for 40 min. Results: Epimedin C was completely hydrolyzed to 2?-O-rhamnosyl icariside II, and 93.38% of the product has been transferred into organic phase; moreover, a high conversion rate had been achieved at 91.69% even after the enzyme solution was used for four cycles. In addition, the procedure was much simplified compared with conventional enzymatic hydrolysis. Conclusion: The newly proposed strategy is an efficient and promising approach for the preparation of 2?-O-rhamnosyl icariside II in industrial application. Abbreviations used: EF: Epimedii Folium; ACN: Acetonitrile; MeOH: Methanol; PTFE: Polytetrafluoroethylene; SD: Standard deviation; ILO-ICSC: International Labor Organization-The International Chemical Safety Cards; HMDB: Human Metabolome Database; HSDB: Hazardous Substances Data Bank.