US EPA

7129676 

Journal Article 

Catechin and epicatechin in leaves of Criolla Roja guava 

del Carmen Perez-Perez, E; Castillo Pirela, VV; Gerardo Ortega, J; Sandoval Sanchez, LE; Medina Lozano, DT; Ramirez Villalobos, M; Ettiene Rojas, GR; , 

2020 

1 

Revista de la Facultad de Agronomía
ISSN: 0378-7818
EISSN: 1690-9763 

UNIV ZULIA, FACULTAD AGRONOMIA 

MARACAIBO 

37 

3 

262-279 

WOS:000564798600003 

Flavonoids as catechin and epicatechin, are secondary metabolites produced by plants and responsible for its growth and interaction with other living organisms. For the purpose of determining catechin and epicatechin in leaves of Criolla Roja guava (Psidium guajava L.), using high-performance liquid chromatography, we selected 20 individuals of the same population of guava of the collection of promising cups of the CESID Fruticola y Apicola-CORPOZULIA Germoplasm Bank. The leaf sample (0.125 g) was submitted to acid hydrolysis HCl (1.2 M): methanol (50:50 % v/v). The separation was performed by high-performance liquid chromatography (HPLC) with UV detection, using as stationary phase a column 100RP-18 (250 x 4 mm x 5 mu m) and as a mobile phase trifluoroacetic acid 0.1 %/acetonitrile 87:13 % v/v, to a flow of 1 mL.min(-1) and a wavelength of 280 nm. The variation of the flavonoid content, derived from the effect of the plant type, was greater for catechin. The plant G-15 presented the highest content of catechin (3799,731 +/- 241,829 mg.100 g(-1) sample) and epicatechin (1259,467 +/- 16,292 mg.100 g(-1) sample). The plant L-3 presented the less value for catechin (46.3190 +/- 3.8300 mg.100 g(-1 )sample). The plant 1-2 presented the lowest value of epicatechin (7.3110 +/- 1.8400 mg.100 g(-1) sample). The plant G-15 is significantly different (P<0.05) to the rest of the plants evaluated. This information will allow to establish a baseline on the production of these secondary metabolites under agroecological conditions of the Mara municipality, Zulia state. 

phytochemical; flavonoids; HPLC; antioxidants; Psidium guajava L.