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7131356 
Journal Article 
New tools for the generation of E1- and/or E3-substituted adenoviral vectors 
Lindqvist, C; Schmidt, M; Heinola, J; Danthinne, X; Werth, E; , 
2000 
1 
Gene Therapy
ISSN: 0969-7128
EISSN: 1476-5462 
English 
10680020 
We have designed new vectors for the construction of recombinant adenoviruses containing expression cassettes in the E1 and/or E3 regions. Using a versatile set of restriction enzymes, the cassettes are cloned into small bacterial vectors and subsequently introduced into large plasmids containing the adenoviral sequences. Two positive selection markers facilitate the recovery of a cosmid containing a copy of the sequence of the recombinant adenovirus. The resulting cosmid is transfected into 293 or 911 cells in order to rescue the virus. Importantly, the method does not require any recombination event, either in E. coli or in mammalian cells. The entire procedure can generate viral plaques in 12 days. Gene Therapy (2000) 7, 80-87.