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7131853 
Journal Article 
Metabolism of estrogens and androgens by embryonic tissues of Arctic charr, Salvelinus alpinus 
Khan, MN; Renaud, RL; Leatherland, JF; , 
1997 
Yes 
General and Comparative Endrocrinology
ISSN: 0016-6480
EISSN: 1095-6840 
107 
1 
118-127 
English 
9208311 
This study examines the ability of Arctic charr (Salvelinus alpinus) embryos to metabolize tritiated androstenedione (A4), testosterone (T), 17 beta-estradiol (E2), and estrone (E1) in vitro; the metabolic products were separated by HPLC. A4 was poorly metabolized, with 48 to 64% of the substrate remaining even after 24 hr of incubation. The major metabolites of A4 metabolism are E1 and some other unidentified metabolites. T was mostly converted to A4, along with some reduced steroids, but E2 was a minor metabolite. Further, while E2 was almost exclusively transformed into E1, when E1 was used as the precursor, there was little metabolism; the products of E1 metabolism were small amounts of E1 sulfate, glucuronide, E2, and an unknown metabolite which cochromatographed with reference steroid androstenetrione (also called 11-ketoandrostenedione). It is concluded that in Arctic charr embryos there is preferential expression of a form of 17 beta-hydroxysteroid dehydrogenase resembling the type 2 isozyme of mammals that converts T and E2 to A4 and E1, respectively. 
Androgens/metabolism; Androstenedione/metabolism; Animals; Aromatase/metabolism; Chromatography, High Pressure Liquid; Culture Techniques; Embryo, Nonmammalian/metabolism; Estradiol/metabolism; Estrogens/metabolism; Estrone/metabolism; Glucuronates/metabolism; Testosterone/metabolism; Trout/embryology/metabolism