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7142008 
Journal Article 
[Cholecalciferol Reference Standard (Control 901) of the National Institute of Hygienic Sciences] 
Kakehi, H; Komatsu, H; Tanaka, M; Murai, M; Ishimitsu, S; Okada, S; , 
1991 
0 
Eisei Shikenjo Hokoku / Bulletin of the National Institute of Hygienic Sciences
ISSN: 0077-4715 
Japanese 
1364379 
Cholecalciferol Reference Standard for the Japanese Pharmacopoeia (JP RS) was prepared. The following analytical data were obtained: melting point 85.8 degrees C; UV and IR spectra were in agreement with both of the previous JP RS and USP RS of Cholecalciferol; absorptivity at 265 nm E1%lcm = 476; optical rotation [alpha]20D = + 110.1 degrees; no impurities were detected by TLC and HPLC analyses; assay 100.1% by HPLC against the USP RS. Based on the above results, the raw material was authorized as the Reference Standard of the National Institute of Hygienic Sciences.