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7150170 
Journal Article 
Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing 
Lange, V; Böhme, I; Hofmann, J; Lang, K; Sauter, J; Schöne, B; Paul, P; Albrecht, V; Andreas, JM; Baier, DM; Nething, J; Ehninger, U; Schwarzelt, C; Pingel, J; Ehninger, G; Schmidt, AH; , 
2014 
Yes 
BMC Genomics
ISSN: 1471-2164 
BMC 
LONDON 
English 
24460756 
WOS:000331115000002 
A close match of the HLA alleles between donor and recipient is an important prerequisite for successful unrelated hematopoietic stem cell transplantation. To increase the chances of finding an unrelated donor, registries recruit many hundred thousands of volunteers each year. Many registries with limited resources have had to find a trade-off between cost and resolution and extent of typing for newly recruited donors in the past. Therefore, we have taken advantage of recent improvements in NGS to develop a workflow for low-cost, high-resolution HLA typing.