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7164581 
Journal Article 
The Effect of Inflammatory Status on Butyrate and Folate Uptake by Tumoral (Caco-2) and Non-Tumoral (IEC-6) Intestinal Epithelial Cells 
Couto, MR; Goncalves, P; Catarino, TA; Martel, F; , 
2017 
Cell Journal
ISSN: 2228-5806
EISSN: 2228-5814 
ROYAN INST 
TEHRAN 
96-105 
28580313 
WOS:000402452400011 
Objective: Colorectal cancer (CRC) is the second leading cause of cancer death in occidental countries. Chronic inflammatory bowel disease (crohn's disease and ulcerative colitis) is associated with an increased risk for CRC development. The aim of this work was to investigate the relationship between inflammatory status and absorption of nutrients with a role in CRC pathogenesis.Materials and Methods: In this experimental study, we evaluated the in vitro effect of tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IF-gamma), and acetylsalicylic acid on C-14-butyrate (C-14-BT), H-3-folic acid (H-3-FA) uptake, and on proliferation, viability and differentiation of Caco-2 and IEC-6 cells in culture.Results: The proinflammatory cytokines TNF-alpha and INF-gamma were found to decrease uptake of a low concentration of C-14-BT (10 mu M) by Caco-2 (tumoral) and IEC-6 (normal) intestinal epithelial cell lines. However, the effect of TNF-alpha and INF-gamma in IEC-6 cells is most probably related to a cytotoxic and antiproliferative impact. In contrast, INF-gamma increases uptake of a high concentration (10 mM) of C-14-BT in Caco-2 cells. The anticarcinogenic effect of BT (10 mM) in these cells is not affected by the presence of this cytokine. On the other hand, acetylsalicylic acid stimulates C-14-BT uptake by Caco-2 cells and potentiates its antiproliferative effect. Finally, both TNF-alpha and INF-gamma cause a significant decrease in H-3-FA uptake by Caco-2 cells.Conclusion: The inflammatory status has an impact upon cellular uptake of BT and FA, two nutrients with a role in CRC pathogenesis. Moreover, the anti-inflammatory acetylsalicylic acid potentiates the anticarcinogenic effect of BT in Caco-2 cells by increasing its cellular uptake.