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HERO ID
7169828
Reference Type
Journal Article
Title
Proteomic analysis of rat skeletal muscle submitted to one bout of incremental exercise
Author(s)
Gandra, PG; Valente, RH; Perales, J; Pacheco, AG; Macedo, DV; ,
Year
2012
Is Peer Reviewed?
1
Journal
Scandinavian Journal of Medicine and Science
ISSN:
0905-7188
EISSN:
1600-0838
Publisher
WILEY
Location
HOBOKEN
Page Numbers
207-216
Language
English
PMID
20973830
DOI
10.1111/j.1600-0838.2010.01235.x
Web of Science Id
WOS:000301288100011
Abstract
Exercise can alter gene transcriptional and protein translational rates leading to changes in protein abundance toward adaptation to exercise. We investigated the alterations in protein abundance in skeletal muscle after one bout of an exhaustive exercise through proteomic analysis. Gastrocnemius muscles were sampled from non-exercised control rats and from rats exercised on a treadmill with incremental increases in speed until exhaustion (approximately 30 min). Rats were sacrificed 3 and 24 h after exercise cessation. Two-dimensional gel electrophoresis was performed and spots with a significant alteration in relative volume were identified by mass spectrometry. Six spots presented statistically significant altered abundances after exercise. The spots identified as the metabolic related proteins triosephosphate isomerase 1, glyceraldehyde-3-phosphate dehydrogenase, the β subunit of pyruvate dehydrogenase E(1) and carnitine palmitoyltransferase 2 were all more abundant after exercise. One spot identified as heat shock cognate 70 was also more abundant after exercise. One spot demonstrated a decreased abundance after exercise and was identified as α-actin. These results suggest that a single session of exhaustive incremental exercise in untrained muscle can alter thin filaments synthesis/degradation rate and enhance cytosolic and mitochondrial proteins synthesis. The identified proteins may be important to a general preconditioning of skeletal muscle for subsequent exercise sessions.
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