Health & Environmental Research Online (HERO)


Print Feedback Export to File
7181365 
Journal Article 
Trifluoroethanol effects on helix propensity and electrostatic interactions in the helical peptide from ribonuclease T1 
Myers, JK; Pace, CN; Scholtz, JM; , 
1998 
1 
Protein Science
ISSN: 0961-8368
EISSN: 1469-896X 
7 
2 
383-388 
English 
9521115 
WOS:000072056400019 
Trifluoroethanol (TFE) is often used to increase the helicity of peptides to make them usable as models of helices in proteins. We have measured helix propensities for all 20 amino acids in water and two concentrations of trifluoroethanol, 15 and 40% (v/v) using, as a model system, a peptide derived from the sequence of the alpha-helix of ribonuclease T1. There are three main conclusions from our studies. (1) TFE alters electrostatic interactions in the ribonuclease T1 helical peptide such that the dependence of the helical content on pH is lost in 40% TFE. (2) Helix propensities measured in 15% TFE correlate well with propensities measured in water, however, the correlation with propensities measured in 40% TFE is significantly worse. (3) Propensities measured in alanine-based peptides and the ribonuclease T1 peptide in TFE show very poor agreement, revealing that TFE greatly increases the effect of sequence context. 
alpha-helix; helical propensity; peptide; ribonuclease T-1; TFE; trifluoroethanol 
• PFAS Universe
     Data Source
          Web of Science
          Pubmed
     2,2,2-Trifluoroethanol