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Citation
Tags
HERO ID
7194146
Reference Type
Journal Article
Title
Liprotides made of alpha-lactalbumin and cis fatty acids form core-shell and multi-layer structures with a common membrane-targeting mechanism
Author(s)
Frislev, HS; Jessen, CM; Oliveira, CLP; Pedersen, JanAlireza; Otzen, DE; ,
Year
2016
Is Peer Reviewed?
1
Journal
Biochimica et Biophysica Acta. Proteins and Proteomics
ISSN:
1570-9639
EISSN:
1878-1454
Publisher
ELSEVIER SCIENCE BV
Location
AMSTERDAM
Page Numbers
847-859
PMID
27068540
DOI
10.1016/j.bbapap.2016.04.003
Web of Science Id
WOS:000376706900012
Abstract
alpha-Lactalbumin (aLA) has been shown to form complexes with oleic acid (OA), which may target cancer cells. We recently showed that aLA and several other proteins all form protein-OA complexes called liprotides with a generic structure consisting of a micellar OA core surrounded by a shell of partially denatured protein. Here we report that a heat treatment and an alkaline treatment method both allow us to prepare liprotide complexes composed of aLA and a range of unsaturated fatty acids (FA), provided the FAs contain cis (but not trans) double bonds. All liprotides containing cis-FA form both small and large species, which all consist of partially denatured aLA, though the overall shape of the species differs. Small liprotides have a simple core-shell structure while the larger liprotides are multi-layered, i.e. they have an additional layer of both FA and alA surrounding the outside of the core-shell structure. All liprotides can transfer their entire FA content to vesicles, releasing aLA as monomers and softening the lipid membrane. The more similar to OA, the more efficiently the different FAs induce hemolysis. We conclude that aLA can take up and transfer a wide variety of FA to membranes, provided they contain a cis bond. This highlights liprotides as a general class of complexes where both protein and cis-FA component can be varied without departing from a generic (though sometimes multi-layered) core-shell structure. (C) 2016 Elsevier B.V. All rights reserved.
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