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7213528 
Journal Article 
Benzophenone used as the photochemical reagent for pinpointing C=C locations in unsaturated lipids through shotgun and liquid chromatography-mass spectrometry approaches 
Xu, T; Pi, Z; Song, F; Liu, Shu; Liu, Z; , 
2018 
Yes 
Analytica Chimica Acta
ISSN: 0003-2670
EISSN: 1873-4324 
ELSEVIER SCIENCE BV 
AMSTERDAM 
1028 
32-44 
English 
29884351 
WOS:000434462800004 
Unsaturated lipids exhibit different physiological significances due to the different locations of the carbonecarbon double bond (C = C). Identifying lipid isomers with mass-based methods remains challenging. Xia's group has been successfully employed Paterno-Buchi (PB) reaction, a photochemical reaction with UV irradiation, coupled with tandem mass spectrometry (MS/MS) to identify and quantify unsaturated lipids in complex mixtures. However, the existing PB reagents possess certain demerits. In this regard, a new PB reagent that is compatible with various lipidomic analysis platforms must be screened. In this study, we comprehensively evaluated the conditions of the PB reaction and screened benzophenone as a new PB reagent. Benzophenone possesses unique advantages, such as a relative high PB yield; the PB products could be readily distinguished from the reacted lipids based on the added high molecular weight (182 Da); and the benzophenone does not affect the lipids appearance interval for reversed-phase column separation. Furthermore, we optimized the reaction conditions by using benzophenone as a PB reagent and summarized the molecular formulas of the diagnostic ions according to the fragment rules. The proposed PB method has been implemented in shotgun and LCeMS lip-idomics. To our best knowledge, this work is the first to report the integrated PB reaction with LCeMS lipidomics for identification of lipid isomers. (C) 2018 Elsevier B.V. All rights reserved.