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7233065 
Journal Article 
CD36 and Macrophage Scavenger Receptor A Modulate Foam Cell Formation via Inhibition of Lipid-Laden Platelet Phagocytosis 
Seizer, P; Gawaz, M; May, AE; Schiemann, S; Merz, T; Daub, K; Bigalke, B; Stellos, K; Mueller, I; Stoeckle, C; Mueller, K; , 
2010 
1 
Seminars in Thrombosis and Hemostasis
ISSN: 0094-6176
EISSN: 1098-9064 
THIEME MEDICAL PUBL INC 
NEW YORK 
157-162 
20414830 
WOS:000276642800005 
CD34(+) progenitor cells are a promising source of regeneration in atherosclerosis or ischemic heart disease. However, as recently published, CD34(+) progenitor cells have the potential to differentiate not only into endothelial cells but also into foam cells upon interaction with platelets. The mechanism of platelet-induced differentiation of progenitor cells into foam cells is as yet unclear. In the present study we investigated the role of scavenger receptor (SR)-A and CD36 in platelet-induced foam cell formation. Human CD34(+) progenitor cells were freshly derived from human umbilical veins and were co-incubated with platelets (2 x 10(8)/mL) up to 14 days resulting in large lipid-laden foam cells. Developing macrophages expressed SR-A, CD36, and Lox-1 as measured by fluorescent-activated cell sorting analysis. The presence of a blocking anti-CD36 or anti-SR-A antibody nearly abrogated foam cell formation, whereas anti-Lox-1 did not affect foam cell formation. Consistently blocking either anti-CD36 or anti-SR-A antibody significantly reduced the phagocytosis of lipid-laden platelets by macrophages. We conclude that CD36 and SR-A play an important role in platelet-induced foam cell formation from CD34(+) progenitor cells and thus represent a promising target to inhibit platelet-induced foam cell formation.