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7235001 
Journal Article 
A novel fluorescent probe for ratiometric detection of formaldehyde in real food samples, living tissues and zebrafish 
Yuan, G; Ding, H; Peng, L; Zhou, L; Lin, Q; , 
2020 
Yes 
Food Chemistry
ISSN: 0308-8146
EISSN: 1873-7072 
331 
English 
32540697 
WOS:000560058300015 
Herein, a two-photon (TP) ratiometric fluorescent probe (NpFA) was developed for detecting formaldehyde (FA) in real food samples, living onion tissues and zebrafish by fluorescence resonance energy transfer (FRET) strategy. Specifically, a TP fluorophore as the donor and a FA turn-on naphthalimide fluorophore as the acceptor were connected by a non-conjugated linker to construct the TP-FRET-based NpFA, which exhibited a target-modulated ratiometric fluorescence response to FA rapidly with high selectivity and sensitivity during 65 s, and a large ratio ~5-fold enhancement at I550/I410 after addition of FA, displaying ~60-fold enhancement at 550 nm and a quite low DOC of 5.8 ± 0.2 nM. Moreover, NpFA has a good imaging resolution and depth of deep tissue penetration. Therefore, based on the above results, NpFA has the capability to be a useful tool for investigating FA in real samples application, and we also hope NpFA will further study of the physiological and pathological function of FA. 
Formaldehyde (FA); FRET probe; Fluorescent probe; Two-photon ratiometric imaging