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HERO ID
7245970
Reference Type
Journal Article
Title
The role of FaBG3 in fruit ripening and B. cinerea fungal infection of strawberry
Author(s)
Li, Q; Ji, Kai; Sun, Y; Luo, Hao; Wang, H; Leng, P
Year
2013
Is Peer Reviewed?
1
Journal
Plant Journal
ISSN:
0960-7412
EISSN:
1365-313X
Volume
76
Issue
1
Page Numbers
24-35
Language
English
PMID
23802911
DOI
10.1111/tpj.12272
Web of Science Id
WOS:000324923900003
Abstract
In plants, β-glucosidases (BG) have been implicated in developmental and pathogen defense, and are thought to take part in abscisic acid (ABA) synthesis via hydrolysis of ABA glucose ester to release active ABA; however, there is no genetic evidence for the role of BG genes in ripening and biotic/abiotic stress in fruits. To clarify the role of BG genes in fruit, eight Fa/FvBG genes encoding β-glucosidase were isolated using information from the GenBank strawberry nucleotide database. Of the Fa/FvBG genes examined, expression of FaBG3 was the highest, showing peaks at the mature stage, coincident with the changes observed in ABA content. To verify the role of this gene, we suppressed the expression of FaBG3 via inoculation with Agrobacterium tumefaciens containing tobacco rattle virus carrying a FaBG3 fragment (RNAi). The expression of FaBG3 in FaBG3-RNAi-treated fruit was markedly reduced, and the ABA content was lower than that of the control. FaBG3-RNAi-treated fruit did not exhibit full ripening, and were firmer, had lower sugar content, and were pale compared with the control due to down-regulation of ripening-related genes. FaBG3-RNAi-treated fruit with reduced ABA levels were much more resistant to Botrytis cinerea fungus but were more sensitive to dehydration stress than control fruit. These results indicate that FaBG3 may play key roles in fruit ripening, dehydration stress and B. cinerea fungal infection in strawberries via modulation of ABA homeostasis and transcriptional regulation of ripening-related genes.
Keywords
strawberry fruit ripening; beta-glucosidase gene; FaBG3-RNAi; ABA; B; cinerea fungus; dehydration
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