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HERO ID
7245984
Reference Type
Journal Article
Title
Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
Author(s)
Borges, A; Serra, S; Abreu, A; Saavedra, MJ; Salgado, A; Simoes, M
Year
2014
Is Peer Reviewed?
Yes
Journal
Biofouling
ISSN:
0892-7014
EISSN:
1029-2454
Volume
30
Issue
2
Page Numbers
183-195
Language
English
PMID
24344870
DOI
10.1080/08927014.2013.852542
Web of Science Id
WOS:000329963000005
Abstract
Quorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect biofilm development and antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate - AITC, benzylisothiocyanate - BITC and 2-phenylethylisothiocyanate - PEITC) and six phenolic products (gallic acid - GA, ferulic acid - FA, caffeic acid - CA, phloridzin - PHL, (-) epicatechin - EPI and oleuropein glucoside - OG) were tested. A disc diffusion assay based on pigment inhibition in Chromobacterium violaceum CV12472 was performed. In addition, the mechanisms of QS inhibition (QSI) based on the modulation of N-acyl homoserine lactone (AHLs) activity and synthesis by the phytochemicals were investigated. The cytotoxicity of each product was tested on a cell line of mouse lung fibroblasts. AITC, BITC and PEITC demonstrated a capacity for QSI by modulation of AHL activity and synthesis, interfering the with QS systems of C. violaceum CviI/CviR homologs of LuxI/LuxR systems. The cytotoxic assays demonstrated low effects on the metabolic viability of the fibroblast cell line only for FA, PHL and EPI.
Keywords
biofilms; Chromobacterium violaceum; cytotoxicity; isothiocyanates; phenolics; phytochemicals; quorum sensing inhibition
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