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7252723 
Journal Article 
Effects of Chemical Sympathectomy on the Level of Reproductive Hormones and Mast Cells During Mouse Early Pregnancy 
Dong, Y; Chen, Y; Wang, Z; Zhao, J; , 
2012 
ACADEMIC JOURNALS INC 
NEW YORK 
960-969 
WOS:000308513800005 
To test the hypothesis that the sympathetic nerve plays a crucial role on the level of 17 beta-estradiol, progesterone and the number and activity of mast cells in the uterus during early pregnancy and the sympathectomy mouse was established by 6-hydroxydopamine (6-OHDA) treatment. Levels of 17 beta-estradiol and progesterone in serum were determined by radioimmunoassay and mast cells number by histochemistry and histamine content using fluorospectrophotometry. Compared with the control, the number of embryos decreased by 64.4-69.9% in the treated groups and the level of 17 beta-estradiol was significantly increased at embryonic days (E3) and E9, while it was decreased at El, E5 and E7. The level of progesterone in the treated group was increased during E1-E5, with peak elevation of 2 folds at E3. The number of mast cells was highest at E3 in both the control and treated mouse. However, it was significantly increased in the treated group by 136.03, 24.54 and 177.97% at E5, E7 and E9, respectively. However, the change in histamine content was significantly decreased 7.5-fold at E3, but increased at E7 and E9 in the treated group; it was near the limit of the detection line in the control at E7 and E9. It can be concluded that the sympathetic nerve has an effect on the synthesis and secretion of sex hormones and regulates mast cells in the uterus during early pregnancy, especially in implantation period.