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HERO ID
7269497
Reference Type
Journal Article
Title
Water-immiscible solvents as diluents in reversed-phase liquid chromatography
Author(s)
Loeser, E; Babiak, S; Drumm, P; ,
Year
2009
Is Peer Reviewed?
Yes
Journal
Journal of Chromatography A
ISSN:
0021-9673
EISSN:
1873-3778
Volume
1216
Issue
15
Page Numbers
3409-3412
Language
English
PMID
19237160
DOI
10.1016/j.chroma.2009.02.012
Web of Science Id
WOS:000265159700060
Abstract
It has recently been shown that the use of strong organic solvents as diluent is possible in RPLC, provided that the solvent used as diluent is retained more strongly by the column than the analytes in the sample. In this study, the phenomenon was further studied experimentally using several water-immiscible solvents (ethyl acetate, isopropyl acetate, and methyl isobutyl ketone) and several model analyte compounds. In all cases, analyte peak distortion was minimal provided the analyte eluted earlier than the diluting solvent, in contrast to substantial broadening and distortion when the analyte eluted after the diluting solvent. The potential analytical utility of this approach is discussed, and an example of a practical application is also presented.
Keywords
Band broadening; Diluent; Immiscible; Injection solvent; Solvent strength
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