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7275163 
Journal Article 
Effects of BPF on steroid hormone homeostasis and gene expression in the hypothalamic–pituitary–gonadal axis of zebrafish 
Yang, Q; Yang, X; Liu, J; Ren, W; Chen, Y; Shen, S 
2017 
Yes 
Environmental Science and Pollution Research
ISSN: 0944-1344
EISSN: 1614-7499 
24 
26 
21311-21322 
English 
28741210 
WOS:000409040700043 
Bisphenol F (BPF) has been frequently detected in various environmental compartments, and previous studies found that BPF exhibits similar estrogenic and anti-androgenic effects on the mammalian endocrine system to those of bisphenol A (BPA). However, the potential disrupting effects of BPF on aquatic organisms and the underling disrupting mechanisms have not been investigated. In this study, the potential disrupting mechanisms of BPF on the hypothalamic–pituitary–gonadal (HPG) axis and liver were probed by employing the OECD 21-day short-term fecundity assay in zebrafish. The results show that BPF exposure (1 mg/L) impaired the reproductive function of zebrafish, as exemplified by alterations to testicular and ovarian histology of the treated zebrafish. Homogenate testosterone (T) levels in male zebrafish decreased in a concentration-dependent manner, and 17β-estradiol (E2) levels increased significantly when fish were exposed to 0.1 and 1 mg/L BPF. The real-time polymerase chain reaction was performed to examine gene expression in the HPG axis and liver. Hepatic vitellogenin expression was significantly upregulated in males, suggesting that BPF possesses estrogenic activity. The disturbed hormone balance was enhanced by the significant changes in gene expression along the HPG axis. These alterations suggest that BPF leads to adverse effects on the endocrine system of teleost fish, and that these effects were more prominent in males than in females. 
Environmental Studies--Pollution; BPA analogs; Bisphenol F (BPF); Hypothalamic–pituitary–gonadal (HPG) axis; Zebrafish; Endocrine disrupting chemicals (EDCs); Fecundity; Hypothalamus; Pituitary; Phenols; Xenoestrogens; Vitellogenin; Testosterone; Compartments; Histology; Bisphenol A; Homeostasis; Polymerase chain reaction; Alterations; Endocrine system; Estrogenic activity; Sex differences; Gene expression; Aquatic organisms; Females; Sex hormones; 17β-Estradiol; Danio rerio