Health & Environmental Research Online (HERO)


Print Feedback Export to File
7363406 
Journal Article 
Review 
Nascent RNA analyses: tracking transcription and its regulation 
Wissink, EM; Vihervaara, A; Tippens, ND; Lis, JT 
2019 
1 
Nature Reviews. Genetics
ISSN: 1471-0056
EISSN: 1471-0064 
20 
12 
705-723 
English 
31399713 
WOS:000499133000004 
The programmes that direct an organism's development and maintenance are encoded in its genome. Decoding of this information begins with regulated transcription of genomic DNA into RNA. Although transcription and its control can be tracked indirectly by measuring stable RNAs, it is only by directly measuring nascent RNAs that the immediate regulatory changes in response to developmental, environmental, disease and metabolic signals are revealed. Multiple complementary methods have been developed to quantitatively track nascent transcription genome-wide at nucleotide resolution, all of which have contributed novel insights into the mechanisms of gene regulation and transcription-coupled RNA processing. Here we critically evaluate the array of strategies used for investigating nascent transcription and discuss the recent conceptual advances they have provided.