US EPA

7414238 

Journal Article 

Anther culture 

Sopory, SK; Munshi, M; , 

1996 

145-176 

10.1007/978-94-017-1860-8_9 

http://link.springer.com/10.1007/978-94-017-1860-8_9
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It is more than thirty years since the publication of the first report on anther culture in Datura innoxia by Guha and Maheshwari (1964). After an initial lag of 2–3 years, the activity in this area increased, especially after the publication of the work on anther culture in tobacco by Nitsch and Nitsch (1969). Realizing the importance of this technique in obtaining haploid plants, and, thereby, homozygous diploids in economically important plants, a large number of laboratories in Universities, Research Institutes and Agricultural Universities started adopting this technique to the plant of their choice. Success was achieved in rice (Niizeki and Oono, 1968) and later many other plants Maheshwari et al., 1980, 1982; Vasil 1980; Heberle-Bors, 1985). Behind this success there were sustained efforts to modify the technique and other growth conditions to suit the requirements of plant species or even a clone or a cultivar. Although this is true even today, yet a survey of the published reports reveals that there are some common guidelines which can be adopted to achieve success. In this review we have attempted to highlight these parameters and also, whereever necessary, given specific examples. Besides this, we have tried to put together some of the significant developments that have contributed to our understanding of the physiological and biochemical basis of androgenesis.