US EPA

7416389 

Journal Article 

Electrochemical detection of carbamate pesticides in fruit and vegetables with a biosensor based on acetylcholinesterase immobilised on a composite of polyaniline-carbon nanotubes 

Cesarino, I; Moraes, FC; Lanza, MR; Machado, SA; , 

2012 

Yes 

Food Chemistry
ISSN: 0308-8146
EISSN: 1873-7072 

ELSEVIER SCI LTD 

OXFORD 

135 

3 

873-879 

English 

22953799 

10.1016/j.foodchem.2012.04.147 

WOS:000310396700003 

https://linkinghub.elsevier.com/retrieve/pii/S0308814612008667
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A sensitive electrochemical acetylcholinesterase (AChE) biosensor was successfully developed on polyaniline (PANI) and multi-walled carbon nanotubes (MWCNTs) core-shell modified glassy carbon electrode (GC), and used to detect carbamate pesticides in fruit and vegetables (apple, broccoli and cabbage). The pesticide biosensors were applied in the detection of carbaryl and methomyl pesticides in food samples using chronoamperometry (CA). The GC/MWCNT/PANI/AChE biosensor exhibited detection limits of 1.4 and 0.95μmolL(-1), respectively, for carbaryl and methomyl. These detection limits were below the allowable concentrations set by Brazilian regulation standards for the samples in which these pesticides were analysed. Reproducibility and repeatability values of 2.6% and 3.2%, respectively, were obtained in the conventional procedure. The proposed biosensor was successfully applied in the determination of carbamate pesticides in cabbage, broccoli and apple samples without any spiking procedure. The obtained results were in full agreement with those from the HPLC procedure. 

Biosensor; Carbamate; Carbon nanotubes; Polyaniline; Acetylcholinesterase; Carbamate; Carbamate pesticides; Carbaryl; Core-shell; Detection limits; ELectrochemical detection; Food samples; Fruit and vegetables; Methomyl; Modified glassy carbon electrode; Reproducibilities; Carbon nanotubes; Chemical contamination; Chronoamperometry; Fruits; Glass membrane electrodes; Pesticides; Polyaniline; Vegetables; Biosensors; acetylcholinesterase; carbamic acid; carbaril; carbon; carbon nanotube; methomyl; multi walled nanotube; pesticide; polyaniline; amperometry; apple; article; biosensor; Brazil; broccoli; cabbage; chronoamperometry; composite material; controlled study; electrochemistry; electrode; enzyme immobilization; food analysis; food quality; fruit; gas chromatography; high performance liquid chromatography; limit of detection; maximum allowable concentration; nonhuman; quality control; reproducibility; standard; vegetable; Acetylcholinesterase; Biosensing Techniques; Carbamates; Electrochemistry; Enzymes, Immobilized; Food Contamination; Fruit; Nanotubes, Carbon; Pesticides; Vegetables; Brassica oleracea var. capitata; Brassica oleracea var. italica; Malus x domestica