US EPA

7416424 

Journal Article 

[Matrix effect and control of immunoassay for environmental samples] 

Sheng, JW; He, M; Shi, HC; , 

2012 

Yes 

Huanjing Kexue / Chinese Journal of Environmental Science
ISSN: 0250-3301 

33 

6 

2095-2103 

Chinese 

22946201 

Immunoassay provides very specific, highly sensitive, rapid, and cost-effective analyses for a variety of environmental contaminants. Since the immunoassay detects the environmental samples without pre-treatment, the interferences caused by various matrixes of environmental samples are a major problem, which can greatly affect the detection results. In this paper, based on the enzyme-linked immunosorbent assay (ELISA) for detection of Microcystin-LR (MC-LR), the effect of many kinds of matrixes on ELISA was systematically analyzed, and the corresponding method to control or eliminate the disbennifit effect was proposed. Finally, an integrated and packaged strategy-using the following buffer system as a diluent, i.e. 0.1 mol x L(-1) pH 7.4 PBS (10 x PBS) containing 1% BSA, 0.5% EDTA and 40 g x L(-1) sodium chloride-was given to control the overall matrix effects of environmental samples on ELISA detection. This strategy is also applicable to other kinds of immunoassays for environmental samples. 

Control strategy; Environmental sample; Immunoassay; Matrix effect; Microcystin-LR; microcystin; microcystin LR; article; environmental monitoring; enzyme linked immunosorbent assay; immunoassay; immunology; methodology; water pollutant; Environmental Monitoring; Enzyme-Linked Immunosorbent Assay; Immunoassay; Microcystins; Water Pollutants, Chemical