US EPA

7423021 

Journal Article 

Using fluorescence immunochromatographic test strips based on quantum dots for the rapid and sensitive determination of microcystin-LR 

Sun, J; Li, Y; Pi, F; Ji, J; Zhang, Y; Sun, X; , 

2017 

Yes 

Analytical and Bioanalytical Chemistry
ISSN: 1618-2642
EISSN: 1618-2650 

SPRINGER HEIDELBERG 

HEIDELBERG 

409 

8 

2213-2220 

English 

28108754 

10.1007/s00216-016-0166-9 

WOS:000395057500023 

http://link.springer.com/10.1007/s00216-016-0166-9
Exit
 

A novel immunosensor for the detection of microcystin-LR (MC-LR) was constructed with use of immunochromatographic test strips (ICTS). Quantum dots were chosen to be the fluorescent labels for the immune sensor in ICTS because of their excellent optical and electronic properties with a relatively narrow emission spectrum. The detection sensitivity of the ICTS was related to the concentration of the fluorescent probe and the amount of the MC-LR standards. Under optimal conditions, with MC-LR as the target, the ICTS sensor had a linear range from 0.25 to 5 μg/L, with a correlation coefficient of 0.9901 and a detection limit of 0.1 μg/L. Furthermore, the repeatability of the ICTS was good, and the coefficient of variation was 10%. The ICTS immunosensor allows the reliable detection of MC-LR in water, and has potential in simple, sensitive detection applications. Graphical Abstract A novel method was developed to detect MC-LR using QDs based immunochromatographic test strip. 

Immunochromatographic test strips; Microcystin-LR; Quantum dots; Electronic properties; Emission spectroscopy; Fluorescence; Graphene quantum dots; Immunosensors; Nanocrystals; Coefficient of variation; Correlation coefficient; Detection sensitivity; Immunochromatographic; Microcystin-LR; Optical and electronic properties; Sensitive detection; Test strips; Semiconductor quantum dots; microcystin; microcystin LR; quantum dot; devices; fluorescence; immunoaffinity chromatography; limit of detection; polyacrylamide gel electrophoresis; reproducibility; Electrophoresis, Polyacrylamide Gel; Fluorescence; Immunochromatography; Limit of Detection; Microcystins; Quantum Dots; Reproducibility of Results