US EPA

7423394 

Journal Article 

Mechanisms of acaricide resistance in the cattle tick Rhipicephalus (Boophilus) microplus in Sri Lanka 

Janadaree Bandara, KMU; Parakrama Karunaratne, SHP; , 

2017 

1 

Pesticide Biochemistry and Physiology
ISSN: 0048-3575
EISSN: 1095-9939 

ACADEMIC PRESS INC ELSEVIER SCIENCE 

SAN DIEGO 

139 

68-72 

English 

28595924 

10.1016/j.pestbp.2017.05.002 

WOS:000403524300010 

https://linkinghub.elsevier.com/retrieve/pii/S0048357517302055
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High tolerance of ticks to acaricides is increasingly becoming a problem to cattle farmers. Resistance status of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)from two cattle farms of Sri Lanka were determined against different concentrations of pyrethroid permethrin, organophosphate malathion, organochlorine DDT and carbamate propoxur using Larval Packet Test (LPT) as recommended by Food and Agriculture Organization (FAO). Mechanisms of acaricide resistance were studied by conducting biochemical and PCR assays. Tick larvae were tested for the activity levels of acaricide metabolizing enzymes i.e.esterases, glutathione S-transferases (GSTs) and monooxygenases, and for altered target sites i.e.acetylcholinesterase (target site of organophosphates and carbamates) and sodium channel regulatory proteins (target site of pyrethroids and DDT). According to discriminating dosages specified by FAO for ticks both populations were 24-56% resistant to DDT. LC values showed that the both populations were susceptible to permethrin and resistant to malathion. Moderate insensitivity of AChEs and knock-down resistance (kdr) mutations were found as resistance mechanisms. GSTs and monooxygenases were not elevated. The kdr type mutation G72V (G215T in the gene) found in the sodium channel regulatory protein of R. (B.) microplus samples may be responsible for DDT resistance. Systematic and sophisticated insecticide resistance monitoring programmes and a better understanding on the mechanisms which govern resistance development are vital for future tick control programmes. 

Altered acetylcholinesterases; Cattle tick; Insecticide detoxifying enzymes; kdr mutations; Rhipicephalus (Boophilus) microplus;insecticide resistance; acaricide; acetylcholinesterase; chlorphenotane; glutathione transferase; insecticide; mixed function oxidase; organophosphate; pyrethroid; animal; drug effects; genetics; insecticide resistance; metabolism; Rhipicephalus; Sri Lanka; Acaricides; Acetylcholinesterase; Animals; DDT; Glutathione Transferase; Insecticide Resistance; Insecticides; Mixed Function Oxygenases; Organophosphates; Pyrethrins; Rhipicephalus; Sri Lanka