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7424062 
Journal Article 
A comparison across non-model animals suggests an optimal sequencing depth for de novo transcriptome assembly 
Francis, WR; Christianson, LM; Kiko, R; Powers, ML; Shaner, NC; Haddock, SH; , 
2013 
Yes 
BMC Genomics
ISSN: 1471-2164 
BMC 
LONDON 
167 
English 
23496952 
WOS:000318941500001 
The lack of genomic resources can present challenges for studies of non-model organisms. Transcriptome sequencing offers an attractive method to gather information about genes and gene expression without the need for a reference genome. However, it is unclear what sequencing depth is adequate to assemble the transcriptome de novo for these purposes.