US EPA

7424404 

Journal Article 

Effect of hydrogen peroxide on Microcystic aeruginosa: Role of cytochromes P450 

Wang, J; Chen, Z; Chen, H; Wen, Y; , 

2018 

1 

Science of the Total Environment
ISSN: 0048-9697
EISSN: 1879-1026 

ELSEVIER SCIENCE BV 

AMSTERDAM 

626 

211-218 

English 

29335171 

10.1016/j.scitotenv.2018.01.067 

WOS:000428194000022 

https://linkinghub.elsevier.com/retrieve/pii/S0048969718300883
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Cyanobacterial bloom has been rising as a worldwide issue owing to its adverse effects to water quality and ecological health. To solve this problem, hydrogen peroxide (H2O2) has been considered as a potential algaecide because no by-products are generated after treatment and because it kills cyanobacteria selectively. In addition, cytochromes P450 (CYPs) was reported to be related with H2O2, but the roles of CYPs in the regulation of H2O2 in cyanobacteria have yet to be investigated. In this study, the CYPs suicide inhibitor 1-aminobenzotriazole (ABT) was added to the representative cyanobacteria Microcystis aeruginosa (M. aeruginosa) exposed to H2O2. The results showed that CYPs mediates the effects of H2O2 on M. aeruginosa. To be exact, the addition of ABT induced greater inhibitory effects on the growth and higher reactive oxygen species levels in M. aeruginosa comparing to those treated with H2O2 alone. At the same time, photosynthetic parameters significantly decreased, and the content of extracellular microcystins (MCs) increased but the total MCs decreased due to the combined effect of H2O2 and ABT. ABT also intensified the aggregation of Fe, which might explain the effects on photosynthesis and synthesis of MCs. Furthermore, the transcriptional levels of MCs-synthesis genes (mcyA and mcyD) decreased but MCs-release gene (mcyH) increased, and photosynthetic genes (psaB, psbD1 and rbcL) decreased, which confirmed the effects on the MC production/release and electron transport of photosynthesis, respectively. In summary, this study illuminated the mediation role of CYPs in the adverse effects on M. aeruginosa induced by H2O2, thus providing new theoretical basis for the explanation of H2O2 as potential algaecide. 

Cytochromes P450; Hydrogen peroxide; Microcystins; Microcystis aeruginosa; Photosynthesis; Bacteria; Electron transport properties; Genes; Hydrogen peroxide; Oxidation; Photosynthesis; Water quality; Cyanobacterial blooms; Cytochromes P450; Microcystic aeruginosa; Microcystins; Microcystis aeruginosa; Photosynthetic parameters; Reactive oxygen species; Transcriptional levels; Peroxides; 1 aminobenzotriazole; cytochrome P450; hydrogen peroxide; iron; microcystin; reactive oxygen metabolite; cytochrome P450; disinfectant agent; hydrogen peroxide; microcystin; cyanobacterium; cytochrome; hydrogen peroxide; inhibitor; photosynthesis; reactive oxygen species; Article; bacterial gene; bacterial growth; controlled study; electron transport; genetic transcription; growth inhibition; mcyA gene; mcyD gene; mcyH gene; Microcystis aeruginosa; nonhuman; photosynthesis; priority journal; protein function; psaB gene; psbD1 gene; rbcL gene; toxin synthesis; cyanobacterium; metabolism; Microcystis; physiology; Pseudomonas aeruginosa; Cyanobacteria; Microcystis aeruginosa; Cyanobacteria; Cytochrome P-450 Enzyme System; Disinfectants; Hydrogen Peroxide; Microcystins; Microcystis; Photosynthesis; Pseudomonas aeruginosa