US EPA

7426620 

Journal Article 

Toxicological assessment of chemicals using Caenorhabditis elegans and optical oxygen respirometry 

Schouest, K; Zitova, A; Spillane, C; Papkovsky, D; , 

2009 

Yes 

Environmental Toxicology and Chemistry
ISSN: 0730-7268
EISSN: 1552-8618 

28 

4 

791 

English 

19006420 

10.1897/08-083.1 

WOS:000264044700012 

http://doi.wiley.com/10.1897/08-083.1
Exit
 

Oxygen consumption is indicative of an organism's metabolic state, whereby alterations in respiration rate can result from the presence of different stimuli. Here, we develop a novel approach based on quenched fluorescence oxygen sensing and respirometry method for toxicity screening assays using the nematode Caenorhabditis elegans. Previously, C. elegans was established as a useful model in soil and aquatic toxicology studies. For existing toxicology screening approaches with C. elegans, however, the endpoint is lethality. In addition, the assay time frame for the existing approaches is considerably longer than that for the approach described here. We present a sensitive, robust, high-throughput platform using standard laboratory equipment for toxicological studies by measuring respiration rate in C. elegans animals using a phosphorescent probe. 

Optical oxygen respirometry; Oxygen consumption; Quenched fluorescence oxygen sensing; Toxicity screening assay; Aquatic toxicologies; Caenorhabditis elegans; Elegans; High throughputs; Laboratory equipments; Metabolic state; Optical oxygen respirometry; Oxygen consumption; Quenched fluorescence oxygen sensing; Respiration rates; Time frames; Toxicological assessments; Toxicology screenings; Animals; Assays; Fluorescence; Geologic models; Oxygen sensors; Toxicity; Oxygen; bacterial toxin; cadmium; paraoxon; phenol; rotenone; zinc ion; assay; assessment method; metabolism; nematode; oxygen; respiration; toxicity test; toxicology; article; Caenorhabditis elegans; experimental model; fluorescence analysis; high throughput screening; laboratory test; nonhuman; optical oxygen respirometry; oxygen sensing; photochemical quenching; priority journal; respirometry; screening test; toxicity testing; Animals; Cadmium; Caenorhabditis elegans; Dimethyl Sulfoxide; Dose-Response Relationship, Drug; Environmental Monitoring; Environmental Pollutants; Fluorescence; Microcystins; Oxygen Consumption; Paraoxon; Phenols; Reproducibility of Results; Respiration; Risk Assessment; Rotenone; Sensitivity and Specificity; Time Factors; Toxicity Tests; Zinc; Animalia; Caenorhabditis elegans