US EPA

7429052 

Journal Article 

An SPR biosensor for the detection of microcystins in drinking water 

Herranz, S; Bocková, M; Marazuela, MD; Homola, J; Moreno-Bondi, MC; , 

2010 

Yes 

Analytical and Bioanalytical Chemistry
ISSN: 1618-2642
EISSN: 1618-2650 

SPRINGER HEIDELBERG 

HEIDELBERG 

398 

6 

2625-2634 

English 

20532874 

10.1007/s00216-010-3856-8 

WOS:000283588600023 

http://link.springer.com/10.1007/s00216-010-3856-8
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A surface plasmon resonance (SPR) biosensor for the detection of microcystins (MCs) in drinking water has been developed. Several assay formats have been evaluated. The selected format is based on a competitive inhibition assay, in which microcystin-LR (MCLR) has been covalently immobilized onto the surface of an SPR chip functionalized with a self-assembled monolayer. The influence of several factors affecting sensor performance, such as the nature and concentration of the antibody, the composition of the carrier buffer, and the blocking and regeneration solutions, has been evaluated. The optimized SPR biosensor provides an IC(50) 0.67 ± 0.09 µg L(-1), a detection limit of 73 ± 8 ng L(-1), and a dynamic range from 0.2 to 2.0 µg L(-1) for MCLR. Cross-reactivity to other related MCs, such as microcystin-RR (88%) and microcystin-YR (94%), has also been measured. The SPR biosensor can perform four simultaneous determinations in 60 min, and each SPR chip can be reused for at least 40 assay-regeneration cycles without significant binding capacity loss. The biosensor has been successfully applied to the direct analysis of MCLR in drinking water samples, below the provisional guideline value of 1 µg L(-1) established by the World Health Organization for drinking water. 

Label-free biosensor; Microcystin-LR; Self-assembled monolayer; Surface plasmon resonance; Binding capacities; Competitive inhibition; Cross-reactivity; Detection limits; Direct analysis; Drinking water; Dynamic range; Functionalized; Label-free biosensor; Microcystin-LR; Microcystins; Regeneration cycles; Sensor performance; Simultaneous determinations; SPR biosensor; Surface plasmon resonance biosensor; Surface plasmons; World Health Organization; Biosensors; Carrier concentration; Plasmons; Potable water; Self assembled monolayers; Toxic materials; Surface plasmon resonance; microcystin; monoclonal antibody; article; calibration; genetic procedures; immunoassay; immunology; instrumentation; methodology; sensitivity and specificity; standard; surface plasmon resonance; water pollutant; water supply; Antibodies, Monoclonal; Biosensing Techniques; Calibration; Immunoassay; Microcystins; Sensitivity and Specificity; Surface Plasmon Resonance; Water Pollutants, Chemical; Water Supply