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7429467 
Journal Article 
Toxicity and toxins of natural blooms and isolated strains of Microcystis spp. (Cyanobacteria) and improved procedure for purification of cultures 
Shirai, M; Ohtake, A; Sano, T; Matsumoto, S; Sakamoto, T; Sato, A; Aida, T; Harada, K; Shimada, T; Suzuki, M; , 
1991 
Yes 
Applied and Environmental Microbiology
ISSN: 0099-2240
EISSN: 1098-5336 
AMER SOC MICROBIOLOGY 
WASHINGTON 
57 
4 
1241-1245 
English 
1905521 
WOS:A1991FF03900057 
All samples of cyanobacterial blooms collected from 1986 to 1989 from Lake Kasumigaura, Ibaraki Prefecture, Japan, were hepatotoxic. The 50% lethal doses (LD50s) of the blooms to mice ranged from 76 to 556 mg/kg of body weight. Sixty-eight Microcystis cell clones (67 Microcystis aeruginosa and 1 M. viridis) were isolated from the blooms. Twenty-three strains (including the M. viridis strain) were toxic. However, the ratio of toxic to nontoxic strains among the blooms varied (6 to 86%). Microcystins were examined in six toxic strains. Five toxic strains produced microcystin-RR, -YR, and -LR, with RR being the dominant toxin in these strains. Another strain produced 7-desmethylmicrocystin-LR and an unknown microcystin. This strain showed the highest toxicity. Establishment of axenic strains from the Microcystis cells exhibiting extracellularly mucilaginous materials was successful by using a combination of the agar plate technique and two-step centrifugation. 
cyanoginosin; article; bacterial overgrowth; bacterium culture; Cyanobacterium; nonhuman; priority journal; purification; toxicity; Bacteria (microorganisms); Cyanobacteria; Microcystis; Microcystis aeruginosa; Microcystis viridis 
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