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HERO ID
7431289
Reference Type
Journal Article
Title
Prophylaxis and treatment with a monoclonal antibody of tetrodotoxin poisoning in mice
Author(s)
Rivera, VR; Poli, MA; Bignami, GS
Year
1995
Is Peer Reviewed?
1
Journal
Toxicon
ISSN:
0041-0101
EISSN:
1879-3150
Volume
33
Issue
9
Page Numbers
1231-1237
Language
English
PMID
8585093
DOI
10.1016/0041-0101(95)00060-y
Web of Science Id
WOS:A1995RW05600013
Abstract
The ability of a tetrodotoxin (TTX)-specific monoclonal antibody to confer passive protection against lethal TTX challenge was investigated. The monoclonal antibody, T20G10, has an estimated affinity for TTX of approximately 10(-9) M and is about 50-fold less reactive with anhydrotetrodotoxin and unreactive with tetrodonic acid by competitive immunoassay. T20G10 specifically inhibited TTX binding in an in vitro radioligand receptor binding assay, but had no effect on the binding of saxitoxin to the sodium channel on rat brain membranes. In prophylaxis studies, mice were administered T20G10 via the tail vein 30 min prior to i.p. TTX challenge (10 micrograms/kg). Under these conditions, 100 micrograms T20G10 protected 6/6 mice, whereas 3/6 mice were protected with 50 micrograms T20G10. Non-specific control monoclonal antibody did not protect against lethality. Therapy studies simulating oral intoxication were performed with mice given a lethal dose of TTX by gavage in a suspension of non-fat dry milk in phosphate-buffered saline. Death occurred within 25-35 min in 6/6 mice not treated with T20G10. However, 500 micrograms T20G10 administered via the tail vein 10-15 min after oral TTX exposure prevented death in 6/6 mice. Lower doses of mAb conferred less protection.
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