[ADMAdda5]-microcystins in Planktothrix agardhii strain PH-123 (cyanobacteria)--importance for monitoring of microcystins in the environment | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

7431464

Reference Type

Journal Article

Title

[ADMAdda5]-microcystins in Planktothrix agardhii strain PH-123 (cyanobacteria)--importance for monitoring of microcystins in the environment

Author(s)

Laub, J; Henriksen, P; Brittain, SM; Wang, J; Carmichael, WW; Rinehart, KL; Moestrup, O; ,

Year

2002

Is Peer Reviewed?

Yes

Journal

Environmental Toxicology
ISSN: 1520-4081
EISSN: 1522-7278

Volume

Issue

Page Numbers

351-357

Language

English

PMID

12203956

DOI

10.1002/tox.10042

Web of Science Id

WOS:000177297800006

URL

http://doi.wiley.com/10.1002/tox.10042
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Abstract

Two major and two minor microcystins (MCYST) were isolated from a hepatotoxic Danish strain of Planktothrix agardhii (Gomont) Anagnostidis et Komárek by reversed-phase high-performance liquid chromatography. The microcystins were characterized by UV spectroscopy, amino acid analysis, fast atom bombardment mass spectrometry (FABMS), and high-resolution FABMS. The major microcystins were further analysed by collisionally induced tandem electrospray ionization MS. The microcystins were found to be demethylated variants of MCYST-HtyR (homotyrosine-arginine) and MCYST-LR (leucine-arginine). The two major microcystins contained an acetyl-demethyl variant (ADMAdda) of 3-amino-9-acetoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda). This is the first report of [ADMAdda5]-microcystins in Planktothrix. The two [ADMAdda5]-microcystins inhibited protein phosphatase activity but showed low cross-reactivity with antibodies of an enzyme-linked immunosorbent assay (ELISA), emphasizing the potential underestimation of the toxicity of natural blooms dominated by Planktothrix when microcystin content is quantified using only an ELISA.

Keywords

Cyanobacteria; Hepatotoxins; Microcystins; Planktothrix; Protein phosphatase inhibition; Amino acids; Antibodies; High performance liquid chromatography; Ionization; Mass spectrometry; Monitoring; Ultraviolet spectroscopy; Electrospray ionization; Bacteria; acetic acid derivative; arginine; carboxylic acid derivative; cyanoginosin; leucine; phosphoprotein phosphatase; tyrosine; cyanoginosin; cyclopeptide; phosphoprotein phosphatase; cyanobacterium; toxin; amino acid analysis; article; bacterial strain; bacterioplankton; controlled study; cross reaction; Cyanobacterium; demethylation; environmental monitoring; enzyme inhibition; enzyme linked immunosorbent assay; fast atom bombardment mass spectrometry; isolation procedure; liver toxicity; mass spectrometry; nonhuman; priority journal; quantitative analysis; reversed phase high performance liquid chromatography; toxin analysis; toxin structure; ultraviolet spectroscopy; chemistry; Cyanobacterium; drug effect; environmental monitoring; eutrophication; high performance liquid chromatography; Cyanobacteria; Planktothrix; Planktothrix agardhii; Chromatography, High Pressure Liquid; Cyanobacteria; Environmental Monitoring; Enzyme-Linked Immunosorbent Assay; Eutrophication; Microcystins; Peptides, Cyclic; Phosphoprotein Phosphatase