Effects of microcin SF608 and microcystin-LR, two cyanotobacterial compounds produced by Microcystis sp., on aquatic organisms
Wiegand, C; Peuthert, A; Pflugmacher, S; Carmeli, S; ,
Effects of two cyanobacterial compounds, microcin SF608 and microcystin-LR, were investigated on different physiological parameters of two organisms, the water moss, Vesicularia dubyana, and the waterflea, Daphnia magna. Both compounds are produced by Microcystis species. Microcystin-LR is a potent inhibitor of protein phosphatases 1 and 2A, and microcin SF608 inhibits serine proteases. Other effects of microcystin-LR are well documented in the literature, but adverse effects of microcin SF608 have not been investigated as yet. This study compared the effects of both compounds on detoxication enzymes, microsomal and soluble glutathione-S-transferase (m-, sGST); oxygen stress enzymes, glutathione peroxidase (GP-X), and peroxidase (POD); photosynthetic oxygen production and chlorophyll a:chlorophyll b ratio. mGST was inhibited by both compounds in both organisms, significantly by microcin SF608, possibly indirectly by inhibition of that serine protease transforming the mGST to its active form. The sGST of D. magna was inhibited by microcin SF608, but elevated by microcystin-LR, and elevated by both compounds in V. dubyana. The GP-X in D. magna was not altered by microcin SF608, but elevated parallel to the sGST, whereas the POD in V. dubyana was decreased by both. Photosynthetic oxygen production as well as the chlorophyll a/b ratio showed typical stress reactions, a decrease of oxygen production, and an increase of chlorophyll b, caused both by microcin SF608 and by microcystin-LR. Microcin SF608 was not likely to be detoxified via conjugation to glutathione. The effects of microcin SF608 and microcystin-LR demonstrate that the impact of cyanobacteria on other organisms may not only be directly related to the presently known toxins.
Chlorophyll a/b; Cyanobacterial compound; Cyanotoxin; Glutathione peroxidase; Glutathione S-transferase; Microcin SF608; Microcystin-LR; Peroxidase; Phostosynthesis; Chlorophyll; Enzymes; Marine biology; Photosynthesis; Stress reactions; Bacteria; bacterial toxin; chlorophyll a; chlorophyll b; cyanoginosin LR; glutathione peroxidase; glutathione transferase; heat shock protein; microcin sf608; microsome enzyme; oxygen; peroxidase; phosphoprotein phosphatase 1; phosphoprotein phosphatase 2A; phosphoprotein phosphatase inhibitor; serine proteinase; unclassified drug; cyanobacterium; toxicity test; toxin; article; controlled study; Daphnia; detoxification; enzyme activation; enzyme inhibition; glutathione metabolism; Microcystis; moss; nonhuman; photosynthesis; priority journal; toxicity testing; toxin synthesis; Animals; Bacteriocins; Bryopsida; Daphnia; Enzyme Inhibitors; Glutathione Peroxidase; Microcystins; Oxidative Stress; Peptides, Cyclic; Peroxidase; Photosynthesis; Serine Endopeptidases; Toxicity Tests; Bryophyta; Cyanobacteria; Daphnia; Daphnia magna; Microcystis; Microcystis sp.