US EPA

7437020 

Journal Article 

Benthic cyanobacteria (Oscillatoriaceae) that produce microcystin-LR, isolated from four reservoirs in southern California 

Izaguirre, G; Jungblut, AD; Neilan, BA 

2007 

1 

Water Research
ISSN: 0043-1354
EISSN: 1879-2448 

Elsevier Ltd 

41 

2 

492-498 

English 

17126876 

10.1016/j.watres.2006.10.012 

WOS:000244108500026 

https://www.proquest.com/scholarly-journals/benthic-cyanobacteria-oscillatoriaceae-that/docview/20309748/se-2?accountid=171501
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Cyanobacteria that produce the toxin microcystin have been isolated from many parts of the world. Most of these organisms are planktonic; however, we report on several microcystin-producing benthic filamentous cyanobacterial isolates from four drinking-water reservoirs in southern California (USA): Lake Mathews, Lake Skinner, Diamond Valley Lake (DVL), and Lake Perris. Some samples of benthic material from these reservoirs tested positive for microcystin by an ELISA tube assay, and all the positive samples had in common a green filamentous cyanobacterium 10-15microm in diameter. Seventeen unialgal strains of the organism were isolated and tested positive by ELISA, and 11 cultures of these strains were found to contain high concentrations of microcystin-LR (90-432microgL(-1)). The cultures were analyzed by protein phosphatase inhibition assay (PPIA) and HPLC with photodiode array detector (PDA) or liquid chromatography/mass spectrometry (LC/MS). Microcystin per unit carbon was determined for six cultures and ranged from 1.15 to 4.15microgmg(-1) C. Phylogenetic analysis of four cultures from Lake Skinner and DVL using cyanobacterial-specific PCR and sequencing of the partial 16S rRNA gene suggested the highest similarity to an unidentified cyanobacterium in the oscillatoriales, and to a Phormidium sp. Morphologically, some of the isolates were similar to Oscillatoria, and others resembled Lyngbya. The significance of these organisms lies in the relative scarcity of known toxin producers among freshwater benthic cyanobacteria, and also as a source of cell-bound microcystin in these reservoirs. 

Cyanobacteria; Cyanotoxins; Lyngbya; Microcystin; Phormidium; Algae; Genes; Potable water; Proteins; Reservoirs (water); RNA; Cyanobacteria; Cyanotoxins; Lyngbya; Microcystin; Water pollution; cyanoginosin LR; drinking water; Algae; Genes; Potable water; Proteins; Reservoirs (water); RNA; Water pollution; bioassay; cyanobacterium; drinking water; liquid chromatography; mass spectrometry; phylogenetics; phytobenthos; polymerase chain reaction; reservoir; toxin; article; bacterial strain; bacterium culture; benthos; Cyanobacterium; enzyme inhibition; enzyme linked immunosorbent assay; gene sequence; high performance liquid chromatography; liquid chromatography; Lyngbya; mass spectrometry; morphology; nonhuman; nucleotide sequence; phylogeny; priority journal; statistical significance; unindexed sequence; United States; water supply; California; North America; United States; Cyanobacteria; Lyngbya; Oscillatoria; Oscillatoriaceae; Oscillatoriales; Phormidium; Phormidium sp.; uncultured cyanobacterium