US EPA

7442204 

Journal Article 

Development of an automated system for the quick production of N-13-labeled compounds with high specific activity using anhydrous [N-13]NH3 

Suzuki, K; Yoshida, Y; Shikano, N; Kubodera, A 

1999 

Yes 

Applied Radiation and Isotopes
ISSN: 0969-8043 

Elsevier Science Ltd 

United Kingdom 

50 

6 

1033-1038 

English 

10.1016/S0969-8043(98)00178-X 

WOS:000079835300009 

https://linkinghub.elsevier.com/retrieve/pii/S096980439800178X
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An automated system was developed to synthesize 13N-labeled compounds with high specific activity using anhydrous [13N]NH3 as a synthetic precursor. This system enabled (1) production of an aqueous solution of [13N]NH3, (2) concentration and desiccation of the [13N]NH3 solution, (3) reaction of anhydrous [13N]NH3 with substrate, (4) purification and formulation. By the use of this system, [13N]p-nitrophenyl carbamate ([13N]NPC) ready for i.v. injection could be obtained in 5.1 ± 0.1 min at the yield of 3.5 ± 0,4 GBq, specific activity 460 + 55 GBq/gmol, and radiochemical purity > 99% (n = 3) by irradiating a 10 mM ethanol solution with 18 MeV protons at 17 gA for 25 min. With special precautions, [13N]NPC could be obtained at the extremely high specific activity of 1800 ± 200 GBq/μmol. omated system was developed to synthesize 13N-labeled compounds with high specific activity using anhydrous [13N]NH3 as a synthetic precursor. This system enabled (1) production of an aqueous solution of [13N]NH3, (2) concentration and desiccation of the [13N]NH3 solution, (3) reaction of anhydrous [13N]NH3 with substrate, (4) purification and formulation. By the use of this system, [13N]p-nitrophenyl carbamate ([13N]NPC) ready for i.v. injection could be obtained in 5.1±0.1 min at the yield of 3.5±0.4 GBq, specific activity 460±55 GBq/μmol, and radiochemical purity >99% (n = 3) by irradiating a 10 mM ethanol solution with 18 MeV protons at 17 μA for 25 min. With special precautions, [13N]NPC could be obtained at the extremely high specific activity of 1800±200 GBq/μmol. 

nitrogen-13; anhydrous [N-13]NH3; N-13-labeled compounds; specific activity; automation; radiosynthesis