US EPA

7445463 

Journal Article 

Synthesis, Anticholinesterase, Antioxidant, and Anti-Aflatoxigenic Activity of Novel Coumarin Carbamate Derivatives 

Kurt, BZ; Gazioglu, I; Kandas, N; Sonmez, F 

2018 

ChemistrySelect
ISSN: 2365-6549 

Wiley-Blackwell 

3 

14 

3978-3983 

English 

10.1002/slct.201800142 

WOS:000430386800016 

New coumarin derivatives with the carbamate moiety were synthesized and their inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were evaluated. 4-methyl-2-oxo-2H-chromen-7-yl cycloheptylcarbamate (4 h) showed the strongest inhibition against AChE with IC50 values of 2.30 μM, and 2-oxo-2H-chromen-7-yl-(cyclohexylmethyl)carbamate (4 c) and 4-methyl-2-oxo-2H-chromen-7-yl-(cyclohexylmethyl)carbamate (4 g) were found to be the most potent BuChE inhibitors with IC50 value of 0.003 μM and 0.004 μM, respectively. Moreover antioxidant, anti-aflatoxigenic activities, protective effects against aflatoxin-B1 (AFB1) in H4IIE−C3 cells and effects on glutathione s-transferase of the synthesized compounds were investigated. The synthesized coumarin carbamates inhibited AFB1 in H4IIE−C3 cells. Western blot analyses confirmed that GSTα protein was induced in cells treated with coumarin carbamates and AFB1. These results showed that the synthesized coumarin carbamates possess a potent protective effect against AFB1. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim 

Aflatoxin B1; antioxidant activity; carbamate; coumarin; glutathione-s-transferase; western blot