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Citation
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HERO ID
7446841
Reference Type
Journal Article
Title
Aptamer-based fluorescent sensor array for multiplexed detection of cyanotoxins on a smartphone
Author(s)
Li, Z; Zhang, S; Yu, T; Dai, Z; Wei, Q
Year
2019
Is Peer Reviewed?
Yes
Journal
Analytical Chemistry
ISSN:
0003-2700
EISSN:
1520-6882
Publisher
NLM (Medline)
Volume
91
Issue
16
Page Numbers
10448-10457
Language
English
PMID
31192585
DOI
10.1021/acs.analchem.9b00750
Web of Science Id
WOS:000482545300018
Abstract
Developing easy-to-use and miniaturized detectors is essential for in-field monitoring of environmentally hazardous substances, such as the cyanotoxins. We demonstrated a differential fluorescent sensor array made of aptamers and single-stranded DNA (ssDNA) dyes for multiplexed detection and discrimination of four common cyanotoxins with an ordinary smartphone within 5 min of reaction. The assay reagents were preloaded and dried in a microfluidic chip with a long shelf life over 60 days. Upon the addition of analyte solutions, competitive binding of cyanotoxin to the specific aptamer-dye conjugate occurred. A zone-specific and concentration-dependent reduction in the green fluorescence was observed as a result of the aptamer conformation change. The aptasensors are fully optimized by quantification of their dissociation constants, tuning the stoichiometric ratios of reaction mixtures, and implementation of an internal intensity correction step. The fluorescent sensor array allowed for accurate identification and measurement of four important cyanotoxins, including anatoxin-a (ATX), cylindrospermopsin (CYN), nodularin (NOD), and microcystin-LR (MC-LR), in parallel, with the limit of detection (LOD) down to a few nanomolar (<3 nM), which is close to the World Health Organization's guideline for the maximum concentration allowed in drinking water. The smartphone-based sensor platform also showed remarkable chemical specificity against potential interfering agents in water. The performance of the system was tested and validated with real lake water samples that were contaminated with trace levels of individual cyanotoxins as well as binary, ternary, and quaternary mixtures. Finally, a smartphone app interface has been developed for rapid on-site data processing and result display.
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Harmful Algal Blooms- Health Effects
April 2021 Literature Search
PubMed
WOS
Scopus
Anatoxins
WOS
PubMed
Nodularins
PubMed
WOS
Microcystins
Date Limited
PubMed
WOS
Not Date Limited
PubMed
WOS
Cylindrospermopsin
Date Limited
PubMed
WOS
Not Date Limited
PubMed
WOS
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