US EPA

7447243 

Journal Article 

Design, synthesis, and in vitro evaluation of novel 1,3,4-oxadiazolecarbamothioate derivatives of Rivastigmine as selective inhibitors of BuChE 

Castro, PP; Siqueira, RP; Conforte, L; Franco, CHJ; Bressan, GC; Amarante, GW; Fallah, A; Mohanazadeh, F; Safavi, M 

2020 

1 

Medicinal Chemistry Research
ISSN: 1054-2523
EISSN: 1554-8120 

Springer 

29 

3 

341-355 

English 

10.1007/s00044-019-02475-6 

WOS:000511663800001 

Rivastigmine has been prescribed for the therapy of Alzheimer’s disease (AD) symptoms. This drug is classified in the carbamate derivative group that has dual activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). According to the structure of Rivastigmine and its performance, a new series of 5-aryl-1,3,4-oxadiazole-2-carbamothioate compounds I–XI was synthesized using structure-based drug discovery approaches. For this purpose a set of these compounds were designed with computational docking method and their interactions with amino acid residues in the active sites of AChE and BuChE checked out. The structures of synthesized compounds were established by physicochemical and spectroscopic methods. The carbamoyl moiety of Rivastigmine structure was modified to carbamothioate and the effects of 1,3,4-oxadiazole heterocycle as a pharmacophoric nucleus were investigated. The potential of the synthesized compounds I–XI was evaluated against two most known agents of AD (AChE and BuChE) to determine their IC50 values. The results of the docking showed the range of binding affinity for the best poses of ten individual conformers for any compounds (I–XI) was between −7.81 (VI) and −6.75 (II) kcal/mol. The results of biological experiments displayed that most synthetic compounds (I–VIII) showed moderate to excellent selective activity range against BuChE (0.51–69.44 µM). In vitro cytotoxicity evaluation of these compounds (I–XI) by MTT assay on human dermal fibroblast (HDF) cell line exhibited no activity against HDF. The compound VI [S-(5-(p-tolyl)-1,3,4-oxadiazol-2-yl) ethyl(methyl)carbamothioate] showed the most stable binding affinity (−7.81 kcal/mol) and the lowest IC50 value (0.51 µM) in comparison with Rivastigmine with 7.72 µM and Donepezil with 5.20 µM against BuChE. © 2019, Springer Science+Business Media, LLC, part of Springer Nature. 

1; 3; 4-oxadiazole; Carbamothioate; Rivastigmine; Acetylcholinesterase; Butyrylcholinesterase; Selective inhibitor