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Citation
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HERO ID
7447524
Reference Type
Journal Article
Title
Large-Scale Extraction and Purification of Microcystin-LR by Macroporous Resins
Author(s)
Cao, YQ; Wei, N; Gong, AJ; Qiu, LN
Year
2014
Is Peer Reviewed?
Yes
Journal
Asian Journal of Chemistry
ISSN:
0970-7077
EISSN:
0975-427X
Publisher
Chemical Publishing Co.
Volume
26
Issue
14
Page Numbers
4206-4210
Language
English
DOI
10.14233/ajchem.2014.16069
Web of Science Id
WOS:000344361100013
Abstract
An effective method for large-scale extraction and purification of microcystin-LR (MC-LR) was established. 660 mL supernatant centrifugated from algae serum was absorbed by resin D101. The impurities on D101 resin were removed by 40 % acetic acid (3 L), deionized water (2.5 L), 20 % acetone (2 L) and 10 % ethanol (2 L) in turn. Thus microcystin-LR on D101 resin was purified. The adsorbed microcystin-LR was eluted from the resin with 70 % methanol (9 L). The eluent was concentrated by rotary evaporation at 80 °C. The quality of microcystin-LR in the crude extract was 2075.5 μg. The recovery rate was 79.71 %. In addition, the method of getting microcystin-LR by preparative chromatography is established. The purity of the product was 95 %.
Keywords
Microcystin-LR; Macroporous resin; Extraction; Purification
Tags
Other
•
Harmful Algal Blooms- Health Effects
April 2021 Literature Search
WOS
Scopus
Microcystins
Date Limited
WOS
Not Date Limited
WOS
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