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7447524 
Journal Article 
Large-Scale Extraction and Purification of Microcystin-LR by Macroporous Resins 
Cao, YQ; Wei, N; Gong, AJ; Qiu, LN 
2014 
Yes 
Asian Journal of Chemistry
ISSN: 0970-7077
EISSN: 0975-427X 
Chemical Publishing Co. 
26 
14 
4206-4210 
English 
WOS:000344361100013 
An effective method for large-scale extraction and purification of microcystin-LR (MC-LR) was established. 660 mL supernatant centrifugated from algae serum was absorbed by resin D101. The impurities on D101 resin were removed by 40 % acetic acid (3 L), deionized water (2.5 L), 20 % acetone (2 L) and 10 % ethanol (2 L) in turn. Thus microcystin-LR on D101 resin was purified. The adsorbed microcystin-LR was eluted from the resin with 70 % methanol (9 L). The eluent was concentrated by rotary evaporation at 80 °C. The quality of microcystin-LR in the crude extract was 2075.5 μg. The recovery rate was 79.71 %. In addition, the method of getting microcystin-LR by preparative chromatography is established. The purity of the product was 95 %. 
Microcystin-LR; Macroporous resin; Extraction; Purification 
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• Harmful Algal Blooms- Health Effects
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