Biochemical responses of the earthworm Eisenia fetida andrei exposed to contaminated artificial soil, effects of carbaryl | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

7450603

Reference Type

Journal Article

Title

Biochemical responses of the earthworm Eisenia fetida andrei exposed to contaminated artificial soil, effects of carbaryl

Author(s)

Ribera, D; Narbonne, JF; Arnaud, C; Saint-Denis, M

Year

2001

Is Peer Reviewed?

Journal

Soil Biology and Biochemistry
ISSN: 0038-0717
EISSN: 1879-3428

Publisher

PERGAMON-ELSEVIER SCIENCE LTD

Location

OXFORD

Volume

Issue

7-8

Page Numbers

1123-1130

Language

English

DOI

10.1016/S0038-0717(01)00035-9

Web of Science Id

WOS:000169257800027

URL

https://linkinghub.elsevier.com/retrieve/pii/S0038071701000359
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Abstract

The aim of this study was to investigate the effects of carbaryl, at different concentrations and exposure times, on the biochemical responses of the earthworm, Eisenia fetida andrei (E. andrei) to: (1) elucidate the mechanisms of action of carbamate compounds; and (2) explore the potential for using these responses as biomarkers to monitor carbamate-contaminated soils or for use in sublethal assays to test chemicals in the laboratory. Thus, earthworms were exposed to increasing concentrations of carbaryl (CA) (12, 25 and 50 mg kg(-1) artificial soil) for different periods of time (2. 7 and 14 days) using the standard soil test method. The activities of the following enzymes were measured: catalase (CAT), acetylcholinesterase (AChE). glutathione reductase (GR), glutathione-S-transferase (GST), methoxyresorufin-O-deethylase (MROD) and NADH (NADH Red) and NADPH (NADPH Red) cytochrome reductases activities. Levels of lipid peroxides (LP), peroxidizable lipids (LPI), total glutathione (total GSH) and the percentage of oxidized glutathione (%GSSG) were also determined. Our results showed that cholinesterases are the main target of carbaryl in E. andrei. Carbaryl also inhibited biotransformation enzyme activities but did not induce oxidative stress. Changes were detected in phase I and acetylcholinesterase activities upon exposure to the lowest dose of carbaryl (12 mg kg(-1)). This demonstrated the sensitivity of these parameters in E. andrei. In addition, discrimination between doses and exposure times was clearer when all the responses were considered rather than a selective choice of biomarkers. This confirms our previous finding that a suite of biochemical responses could be used as a sublethal assay for testing chemicals in the laboratory or for soil contamination surveys. (C) 2001 Elsevier Science Ltd. All rights reserved.

Keywords

earthworm; Eisenia; carbaryl; mechanism of action; biomarkers