Journal Article
Spatial distribution and temporal variation of Microcystis species composition and microcystin concentration in Lake Biwa
Ozawa, K; Fujioka, H; Muranaka, M; Yokoyama, A; Katagami, Y; Homma, T; Ishikawa, K; Tsujimura, S; Kumagai, M; Watanabe, MF; Park, HD
Environmental Toxicology
ISSN: 1520-4081
EISSN: 1522-7278
Spatial and temporal variation in Microcystis species composition and microcystin concentration, quantified by enzyme-linked immunosorbent assay and high-performance liquid chromatography, were investigated during a 3-year period (1998-2000) in the Northern Basin of Lake Biwa. The Northern Basin generally had a concentration of 5 microg L(-1) or less, except at station 1 (Nagahama Bay) from July to October during the study period. The maximum concentration at station 1 was 22.7, 35.9, and 22.0 microg L(-1) in October of 1998, 1999, and 2000, respectively. Eleven species of cyanobacteria were observed: Microcystis aeruginosa, M. ichthyoblabe, M. novacekii, M. wesenbergii, Oscillatoria raciborskii, Anabaena oumiana, A. affinis, A. flos-aquae, A. ucrainica, A. smithii, and A. crassa. Of these, M. aeruginosa and M. wesenbergii were the main components observed. A high concentration of microcystin in the lake water was mostly a result of variation in the relative amount of toxic M. aeruginosa rather than of the total Microcystis cell number. This was supported by the analytical results for isolated strains. Microcystis spp. cell density in the Northern Basin appeared to increase gradually over the course of the study. This is the first study to have surveyed the Northern Basin of Lake Biwa, which supplies drinking water to 14 million people and is the largest lake in Japan.
Horizontal distribution of microcystin; Lake Biwa; Microcystin concentration; Microcystis strain; Seasonal variation of microcystin; Bioassay; Catchments; Cells; Enzymes; High performance liquid chromatography; Immunology; Potable water; Toxicity; Cell density; Cell numbers; Cyanocbacteria; Microsystins; Bacteria; bacterial toxin; chlorophyll a; cyanoginosin; drinking water; lake water; cyanobacterium; spatial distribution; species diversity; temporal distribution; Anabaena; anabaena affinis; anabaena crassa; Anabaena flos-aquae; anabaena oumiana; anabaena smithii; anabaena ucrainica; bacterial count; bacterial strain; bacterium isolation; concentration (parameters); conference paper; Cyanobacterium; ecotoxicity; enzyme linked immunosorbent assay; high performance liquid chromatography; Japan; Microcystis; Microcystis aeruginosa; Microcystis ichthyoblabe; microcystis novacekii; microcystis wesenbergii; nonhuman; oscillatoria raciborskii; priority journal; seasonal variation; species composition; species distribution; species diversity; Chromatography, High Pressure Liquid; Classification; Data Collection; Environmental Monitoring; Enzyme Inhibitors; Japan; Microcystins; Microcystis; Peptides, Cyclic; Population Dynamics; Seasons; Water; Asia; Biwa Lake; Eastern Hemisphere; Eurasia; Far East; Honshu; Japan; Kinki; Shiga; World; Anabaena; Anabaena affinis; Anabaena crassa; Anabaena smithii; Anabaena ucrainica; Cyanobacteria; Microcystis; Microcystis aeruginosa; Microcystis ichthyoblabe; Microcystis novacekii; Microcystis wesenbergii; Planktothricoides raciborskii