Journal Article
Advancing nonviral gene delivery: Lipid- and surfactant-based nanoparticle design strategies
Donkuru, M; Badea, I; Wettig, S; Verrall, R; Elsabahy, M; Foldvari, M
Nanomedicine
ISSN: 1743-5889
EISSN: 1748-6963
Gene therapy is a technique utilized to treat diseases caused by missing, defective or overexpressing genes. Although viral vectors transfect cells efficiently, risks associated with their use limit their clinical applications. Nonviral delivery systems are safer, easier to manufacture, more versatile and cost effective. However, their transfection efficiency lags behind that of viral vectors. Many groups have dedicated considerable effort to improve the efficiency of nonviral gene delivery systems and are investigating complexes composed of DNA and soft materials such as lipids, polymers, peptides, dendrimers and gemini surfactants. The bottom-up approach in the design of these nanoparticles combines components essential for high levels of transfection, biocompatibility and tissue-targeting ability. This article provides an overview of the strategies employed to improve in vitro and in vivo transfection, focusing on the use of cationic lipids and surfactants as building blocks for nonviral gene delivery systems. © 2010 Future Medicine Ltd.
active targeting; DNA; gemini surfactant; gene therapy; lipoplexes; nonviral delivery systems; soft nanoparticles; 1,2 dipalmitoyl sn glycero 3 phosphoethanolamidospermine; 2,3 dioleyloxy n [2[sperminecarboxyamido]ethyl] n,n dimetyl 1 propaniminium bromide; 3beta [n (n',n' dimethylaminoethane)carbamoyl)cholesterol; bis(guanidinium) tren cholesterol; bis[2 (11 butyloxyundecanoate)ethyl]dimethylammonium bromide; bis[2 (11 phenoxyundecanoate)ethyl]dimethylammonium bromide; bis[n [1 (2,3 dicetyloxy)propyl]] n,n,n'n' tetramethyl 1,3 propanediammonium chloride; cetrimide; dendrimer; dimethyldioctadecylammonium bromide; dimethyldioctadecylammonium chloride; dimyristyloxypropyl 3 dimethylhydroxyethylammonium bromide; DNA vaccine; interleukin 12; lipid; lipofectamine; lipoplex; n (3 aminopropyl) n,n dimethyl 2,3 bis(dodecyloxy) 1 propanimidium bromide; n [1 (2,3 dioleoyloxy)propyl] n,n,n trimethylammonium chloride; n [1 (2,3 dioleyloxy)propyl] n,n,n trimethylammonium chloride; n hexadecyl n [10 [o (4 acetoxy)phenylundecanoate]ethyl]dimethylammonium bromide; n,n,n'n' tetraoleyl n,n' dimethyl 1,3 propanediammonium chloride; naked DNA; nanoparticle; o [1,2 di o[1',9' octadecadienyl]glycerol] 3 n(bis 2 aminoethyl)carbamate; peptide; plasmid DNA; polymer; surfactant; unclassified drug; unindexed drug; animal cell; biocompatibility; breast cancer; cancer survival; clinical trial; coronary artery disease; cystic fibrosis; DNA binding; drug design; drug formulation; drug safety; drug targeting; drug tolerability; endocytosis; gene expression; genetic transfection; head and neck cancer; human; lung cancer; melanoma; nonhuman; nonviral gene delivery system; ovary cancer; pancreas cancer; particle size; priority journal; prostate cancer; review; structure activity relation; virus infection; Gene Transfer Techniques; Ligands; Lipids; Nanoparticles; Surface-Active Agents