US EPA

7456653 

Journal Article 

农田水样中微囊藻毒素-LR 拉曼检测方法的研究 

Huang, S; Meng, H; Zeng, K; Huang, Z 

2020 

1 

Nongye Huanjing Kexue Xuebao / Journal of Agro-Environment Science
ISSN: 1672-2043 

Editorial Board of Journal of Agro-Environment Science 

39 

8 

1862-1868 

Chinese 

10.11654/jaes.2020-0341 

In this study, a surface-enhanced Raman scattering（SERS）method based on spiny gold nanoparticles（SGNPs）was developed for detecting microcystin-LR（MC-LR） in irrigation water samples. SGNPs were prepared by the media growth method and then characterized by ultraviolet-visible（UV-vis）absorbance spectra and transmission electron microscopy（TEM）. The SERS performances of SGNPs were comparatively evaluated using 4-mercaptobenzoic acid（4-MBA）as a signal reporter. According to the experimental results, the study chose Au TNs as the SERS detection base to establish a SERS method for detecting MC-LR in irrigation water samples. The characteristic peaks of MC-LR at 1 007 cm-1 and 1 309 cm-1 were chosen as the quantitative peaks. This method was performed with an excitation laser wavelength of 785 nm and the integration time for each spectrum was set to 20 s. The calibration curved showed a linear detection range from 1 µg·L-1 to 100 000 µg·L-1. The limit of detection（LOD）and recoveries of this method were 1.0 µg·L-1 and 80%~ 102%, respectively. The results of this method using naturally irrigation water samples showed that the concentrations of MC-LR were higher than 1 µg·L-1 in 40% of the samples, with the highest level reaching 1.81 µg·L-1. The correlation coefficient was 0.84 when compared to LC-MS/MS. Therefore, the SERS method based on SGNPs could meet the requirements of fast and on-site screening of MC-LR in irrigation water samples. © 2020 Editorial Board of Journal of Agro-Environment Science. All rights reserved. 

Microcystin-LR; Spiny gold nanoparticles; Surface-enhanced Raman scattering