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7463652 
Journal Article 
Phytoplankton profile and toxicity assessment of dominant algal species from different Egyptian aquatic ecosystems 
Marrez, DA; Naguib, MM; Sultan, YY; Daw, ZY; Zaher, SS; Higazy, AM 
2016 
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 
1452-1461 
English 
A total of 98 species of phytoplankton from 4 different Egyptian sites Kafr El-Zayat, El-Rasua, Wadi El-Rayian Lake and El-Khadra were identified to specify the dominant species. Six dominant species were isolated and purified, four of them (Oscillatoria brevis, O. princeps, Microcystis aeruginosa and Spirulina platensis) were belonged to Cyanobacteria and the other two (Chlorella vulgaris and Scenedesmus obliquus) were belonged to green algae. The toxicity of these species was assessed using brine shrimp and mouse bioassays. The extract of Oscillatoria brevis and Microcystis aeruginosa exhibited a toxic effect against both bioassays. This result was confirmed by the determination of microcystin-LR using HPLC. High quantities of microcystin-LR were detected in M. aeruginosa (1.87μg g-1 dry weight) and O. brevis (0.81μg g-1 dry weight). 
phytoplankton; brine shrimp; mouse; bioassay; microcystin-LR